Paraffin Embedding and Thin Sectioning of Microbial Colony Biofilms for Microscopic Analysis

Preparation of Adult Drosophila Eyes for Thin Sectioning and Microscopic Analysis

Drosophila has long been used as model system to study development, mainly due to the ease with which it is genetically tractable. Over the years, a plethora of mutant strains and technical tricks have been developed to allow sophisticated questions to be asked and answered in a reasonable amount of time. Fundamental insight into the interplay of components of all known major signaling pathways...

Thin Sectioning of Slice Preparations for Immunohistochemistry

Many investigations in neuroscience, as well as other disciplines, involve studying small, yet macroscopic pieces or sections of tissue that have been preserved, freshly removed, or excised but kept viable, as in slice preparations of brain tissue. Subsequent microscopic studies of this material can be challenging, as the tissue samples may be difficult to handle. Demonstrated here is a method ...

architecture and engineering of nanoscale sculptured thin films and determination of their properties

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Thin Sectioning: Details of Techniques

The study of cytological fine structure with the electron microscope, has as an important prerequisite, the ability to cut consistently good quality thin sections. Satisfactory techniques for doing this are well known in many laboratories and an excellent review of the subject has recently been published by Farquhar (1). However, some of the details of the practice of microtomy are not in the l...

Microbial Biofilms